7Y9C

Crystal structure of METTL9 in complex with SLC39A5 peptide and SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	I 1 2 1
Unit cell lengths	106.280, 46.046, 124.780
Unit cell angles	90.00, 103.35, 90.00

Refinement procedure

Resolution	31.829 - 2.100
R-factor	0.1945
Rwork	0.192
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	model from alphafold2
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.050	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	34530	34530
<I/σ(I)>	14.6
Completeness [%]	99.6
Redundancy	6.4
CC(1/2)	0.998	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M MES monohydrate pH6.5, 12 % w/v PEG 20000, 0.2 M sodium thiocyanate