7Y96
Crystal structure of the carboxy-terminal domain of a coronavirus M protein fused with a split GFP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 82.020, 82.020, 427.841 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.292 - 3.415 |
R-factor | 0.2409 |
Rwork | 0.240 |
R-free | 0.26460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3q |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.330 | 46.330 | 3.462 |
High resolution limit [Å] | 3.400 | 9.437 | 3.400 |
Rmerge | 0.150 | 0.041 | 0.563 |
Rmeas | 0.169 | 0.047 | 0.632 |
Rpim | 0.077 | 0.022 | 0.280 |
Total number of observations | 52103 | 2361 | 2698 |
Number of reflections | 11907 | 595 | 596 |
<I/σ(I)> | 8.9 | 23 | 2.7 |
Completeness [%] | 93.1 | 80.2 | 93.9 |
Redundancy | 4.4 | 4 | 4.5 |
CC(1/2) | 0.990 | 0.997 | 0.821 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 293 | 0.4 M ammonium sulfate, 0.1 M Bis-Tris pH 5.3, PEG 3350 27%, 0.5% ethyl acetate |