7Y7P

QDE-1 in complex with RNA template, RNA primer and AMPNPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97928
Spacegroup name	P 1 21 1
Unit cell lengths	101.949, 120.201, 114.857
Unit cell angles	90.00, 109.62, 90.00

Refinement procedure

Resolution	29.620 - 2.700
R-factor	0.2242
Rwork	0.223
R-free	0.25420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j7n
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.800
High resolution limit [Å]	2.700	5.800	2.700
Rmerge	0.063	0.039	0.333
Rmeas	0.070	0.043	0.390
Rpim	0.029	0.016	0.196
Total number of observations	337787
Number of reflections	70175	7270	6751
<I/σ(I)>	7.9
Completeness [%]	98.1	99.8	94.4
Redundancy	4.8	6.4	3.2
CC(1/2)		0.999	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	291	PEG 4000, 200 mM KCl