7Y5O
Crystal structure of human CAF-1 core complex in spacegroup P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.613, 176.092, 100.913 |
| Unit cell angles | 90.00, 115.09, 90.00 |
Refinement procedure
| Resolution | 26.980 - 3.570 |
| R-factor | 0.2153 |
| Rwork | 0.214 |
| R-free | 0.23210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7y5k |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.464 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20_4459: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.730 |
| High resolution limit [Å] | 3.570 | 3.600 |
| Rmerge | 0.110 | 0.584 |
| Number of reflections | 56505 | 2062 |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 7% Tacsimate, pH 6.0, 18% (w/v) PEG 3350, 0.12 M lithium citrate and 0.13 M sodium tartrate |
