7Y1I
Crystal Structure of TTR-G67R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 31 |
| Unit cell lengths | 140.982, 140.982, 165.287 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.150 - 2.790 |
| R-factor | 0.2745 |
| Rwork | 0.272 |
| R-free | 0.31390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n85 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.719 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.980 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.198 | 0.796 |
| Rpim | 0.069 | 0.516 |
| Number of reflections | 101651 | 4059 |
| <I/σ(I)> | 9.7 | 1.56 |
| Completeness [%] | 99.6 | 95.4 |
| Redundancy | 10.5 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | AMMONIUM SULFATE, PEG3350 |
