7XWZ
Crystal structure of SARS-CoV-2 N-NTD and dsRNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-27 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.18057 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.848, 39.119, 84.157 |
Unit cell angles | 90.00, 97.61, 90.00 |
Refinement procedure
Resolution | 47.977 - 2.250 |
Rwork | 0.226 |
R-free | 0.26850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ofz |
RMSD bond length | 0.003 |
RMSD bond angle | 1.287 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.977 | 2.320 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.049 | 0.432 |
Number of reflections | 16782 | 1511 |
<I/σ(I)> | 17.7 | |
Completeness [%] | 99.6 | |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | Malonic Acid, Imidazole, Boric Acid, PEG 1500 |