7XT2
Crystal structure of TRIM72
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.571, 125.231, 174.537 |
| Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
| Resolution | 43.140 - 3.000 |
| R-factor | 0.2206 |
| Rwork | 0.219 |
| R-free | 0.25470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kb5 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20rc1_4392) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.140 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.097 | |
| Rpim | 0.040 | |
| Number of reflections | 60046 | 3064 |
| <I/σ(I)> | 13.14 | |
| Completeness [%] | 98.6 | 99 |
| Redundancy | 6.9 | |
| CC(1/2) | 0.999 | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | PEG8000 |
