7XQK

The Crystal Structure of CDK3 and CyclinE1 Complex from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.18078
Spacegroup name	P 63
Unit cell lengths	154.154, 154.154, 76.675
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.500 - 2.250
Rwork	0.160
R-free	0.20240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eoj
RMSD bond length	0.008
RMSD bond angle	1.494
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.500	2.320
High resolution limit [Å]	2.250	2.250
Rmerge	0.139	0.973
Number of reflections	49467	4563
<I/σ(I)>	15.9	2.7
Completeness [%]	100.0
Redundancy	10.2
CC(1/2)	0.998	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.6M MgSO4, 0.1M MES pH6.5