7XPM
Ancestral ADH WT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 150 |
| Detector technology | PIXEL |
| Collection date | 2021-12-16 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.725, 51.792, 74.220 |
| Unit cell angles | 90.00, 128.35, 90.00 |
Refinement procedure
| Resolution | 22.300 - 2.350 |
| R-factor | 0.2204 |
| Rwork | 0.218 |
| R-free | 0.26030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zed |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.360 | 2.440 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.117 | 0.401 |
| Number of reflections | 14409 | 1500 |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 289.2 | PEG 2000, TRIS hydrochloride |
