7XMN
Structure of SARS-CoV-2 ORF8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 140.088, 146.693, 74.718 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.130 - 2.300 |
R-factor | 0.2164 |
Rwork | 0.215 |
R-free | 0.24530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3set |
RMSD bond length | 0.003 |
RMSD bond angle | 0.570 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 101.310 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 33261 | 3311 |
<I/σ(I)> | 7.9 | |
Completeness [%] | 96.8 | 99.2 |
Redundancy | 4.4 | 4.5 |
CC(1/2) | 0.989 | 0.726 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M MES pH6.1, 2%Polyethylene glycol 400, 2.1 M Ammonium sulfate |