7XKA
Structure of human beta2 adrenergic receptor bound to constrained epinephrine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-07-15 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.600, 66.560, 302.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 3.100 |
| R-factor | 0.2309 |
| Rwork | 0.226 |
| R-free | 0.27240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lde |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.539 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 18898 | 1618 |
| <I/σ(I)> | 6.73 | |
| Completeness [%] | 99.0 | 97.2 |
| Redundancy | 11.3 | |
| CC(1/2) | 0.983 | 0.598 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 100mM Tris-HCl, pH 8.0, 150-200mM lithium acetate, 43-45% PEG400 |
