7XE3

Crystal structure of LSD2 in complex with cis-4-Br-2,5-F2-PCPA (S1024)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-16
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.9190
Spacegroup name	I 2 2 2
Unit cell lengths	143.372, 171.226, 201.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.270 - 2.820
R-factor	0.179
Rwork	0.177
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fwe
RMSD bond length	0.007
RMSD bond angle	0.849
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.270	2.890
High resolution limit [Å]	2.820	2.820
Number of reflections	60041
<I/σ(I)>	11.9	1.6
Completeness [%]	100.0
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	330mM Ammonium citrate, 26% PEG 3350