7XE1
Crystal structure of LSD2 in complex with cis-4-Br-PCPA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-13 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9195 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 144.121, 170.760, 202.669 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.750 - 2.070 |
| R-factor | 0.166 |
| Rwork | 0.164 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fwe |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.899 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.390 | 2.110 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Number of reflections | 151257 | |
| <I/σ(I)> | 21.4 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.4 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 320mM Ammonium citrate, 22% PEG 3350 |
