7X88

Crystal structure of ENL YEATS domain T2 mutant in complex with histone H3 acetylation at K27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-15
Detector	MAR CCD 130 mm
Wavelength(s)	0.9789
Spacegroup name	P 31 2 1
Unit cell lengths	52.141, 52.141, 136.394
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.160 - 2.250
R-factor	0.2125
Rwork	0.211
R-free	0.24030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j9s
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.290
High resolution limit [Å]	2.250	6.100	2.250
Rmerge	0.132	0.076	0.735
Rmeas	0.136	0.079	0.775
Rpim	0.034	0.023	0.235
Total number of observations	163971
Number of reflections	10777	629	489
<I/σ(I)>	8.2
Completeness [%]	99.5	98.9	94.6
Redundancy	15.2	12.6	7.7
CC(1/2)		0.997	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	291	20% (w/v) PE G3350, 0.1M sodium citrate/citric acid pH 4.0, 0.2M sodium citrate tribasic