7X5Q

Apo Truncated VhChiP (Delta 1-19) in complex with peptide (DGANSDAAK)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	110
Detector technology	CCD
Collection date	2020-08-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	85.085, 182.602, 196.808
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.870 - 2.700
R-factor	0.2128
Rwork	0.210
R-free	0.25610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mdo
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.700
High resolution limit [Å]	2.700	2.700
Rmeas	0.331	0.331
Rpim	0.089	0.819
Number of reflections	84382	84382
<I/σ(I)>	14.2	14.2
Completeness [%]	97.3
Redundancy	7.2
CC(1/2)	0.752	0.504

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	292	0.1M Magnesium nitrate hexahydrate, 0.05M Magnesium acetate tetrahydrate, 0.1M Sodium citrate pH 5.0, and 18% w/vPEG 1500