7X2M
Crystal structure of nanobody 1-2C7 with SARS-CoV-2 RBD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.987 |
Spacegroup name | I 2 3 |
Unit cell lengths | 143.619, 143.619, 143.619 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.390 - 1.800 |
R-factor | 0.1809 |
Rwork | 0.180 |
R-free | 0.20720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6m0j |
Data reduction software | HKL-3000 (7.21) |
Data scaling software | HKL-3000 (7.21) |
Phasing software | PHASER (2.7.0) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.390 | 1.864 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 45687 | 4537 |
<I/σ(I)> | 33.27 | 1.57 |
Completeness [%] | 99.9 | |
Redundancy | 38.8 | |
CC(1/2) | 0.998 | 0.602 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2M Ammonium sulfate, 0.1M Bis Tris pH 5.5, 25% w/v Polyethylene glycol 3350 |