7WZC

An open conformation Form2 of switch II for RhoA GDP-bound state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	31.487, 66.358, 83.363
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.681 - 1.799
R-factor	0.207644606462
Rwork	0.205
R-free	0.26407
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ftn
RMSD bond length	0.008
RMSD bond angle	0.892
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.681	1.864
High resolution limit [Å]	1.799	1.799
Rmeas	0.077	0.694
Rpim	0.021	0.201
Number of reflections	16632	1439
<I/σ(I)>	30.3
Completeness [%]	99.0
Redundancy	12.7
CC(1/2)	0.998	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1M MES, pH6.0, 30% PEG 600, 5% PEG 1000, 10% glycerol