7WWG

Crystal structure of Saccharomyces cerevisiae Sfh2 complexed with phosphatidylinositol in an open conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	110
Detector technology	CCD
Collection date	2020-10-30
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	75.137, 75.137, 146.807
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.440 - 3.400
R-factor	0.2447
Rwork	0.240
R-free	0.28250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.403
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.460
High resolution limit [Å]	3.400	3.400
Rmerge	0.106	0.205
Rpim	0.028
Number of reflections	10956	288
<I/σ(I)>	48.8	5.5
Completeness [%]	99.5	100
Redundancy	14.8	15
CC(1/2)	0.993	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.1M MES-NaOH pH 6.0, 30% PEG 1000