7WPV

Fab14 - a SARS-CoV2 RBD neutralising antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-07-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9464
Spacegroup name	C 1 2 1
Unit cell lengths	77.700, 71.220, 96.760
Unit cell angles	90.00, 99.07, 90.00

Refinement procedure

Resolution	48.030 - 2.460
R-factor	0.2126
Rwork	0.210
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c01
RMSD bond length	0.010
RMSD bond angle	1.270
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	2.548
High resolution limit [Å]	2.460	2.460
Rmerge	0.128	1.076
Rmeas	0.139
Rpim	0.052	0.442
Number of reflections	18869	1696
<I/σ(I)>	13.6
Completeness [%]	98.8	89.35
Redundancy	6.9	6.7
CC(1/2)	0.997	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1M Lithium Chloride, 30% PEG 4000