7WPV
Fab14 - a SARS-CoV2 RBD neutralising antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-07-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9464 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.700, 71.220, 96.760 |
| Unit cell angles | 90.00, 99.07, 90.00 |
Refinement procedure
| Resolution | 48.030 - 2.460 |
| R-factor | 0.2126 |
| Rwork | 0.210 |
| R-free | 0.25630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7c01 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.270 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 2.548 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.128 | 1.076 |
| Rmeas | 0.139 | |
| Rpim | 0.052 | 0.442 |
| Number of reflections | 18869 | 1696 |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 98.8 | 89.35 |
| Redundancy | 6.9 | 6.7 |
| CC(1/2) | 0.997 | 0.765 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1M Lithium Chloride, 30% PEG 4000 |
