7WPJ

Methionyl-tRNA synthetase from Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-05
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	59.728, 68.987, 117.404
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.480 - 2.650
R-factor	0.2201
Rwork	0.217
R-free	0.27390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qre
RMSD bond length	0.009
RMSD bond angle	1.155
Data reduction software	CrysalisPro
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.730	2.740
High resolution limit [Å]	2.650	2.650
Rmerge	0.030	0.070
Number of reflections	14046	1402
<I/σ(I)>	17.8	8.7
Completeness [%]	96.0
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	300	sodium cacodylate, PEG 3350