7WKI

Structure of the ultra-affinity complex between CFH and a nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-17
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	75.960, 75.960, 143.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.440 - 2.600
Rwork	0.192
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sw0 5lhn
RMSD bond length	0.012
RMSD bond angle	1.863
Data reduction software	MOSFLM (7.2.2)
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.440	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.160
Number of reflections	15303	2204
<I/σ(I)>	12.3	2.6
Completeness [%]	99.9
Redundancy	13.5
CC(1/2)	0.997	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Sodium Phosphate monohydrate, 20% PEG 3350