7WAG

Crystal structure of MurJ squeezed form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	74.450, 75.060, 115.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.700 - 2.550
R-factor	0.2195
Rwork	0.214
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cc4
RMSD bond length	0.004
RMSD bond angle	0.778
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.700	2.640
High resolution limit [Å]	2.550	2.550
Number of reflections	21690	2154
<I/σ(I)>	10.98
Completeness [%]	99.9	100
Redundancy	35.7
CC(1/2)	0.996	0.502

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293.15	MPD, MgCl2, Tris-HCl, PEG, OLC