7W80

Crystal Structure of the Heterodimeric HIF-2 in Complex with Antagonist Belzutifan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	49.192, 98.187, 77.739
Unit cell angles	90.00, 107.16, 90.00

Refinement procedure

Resolution	46.380 - 2.754
R-factor	0.2221
Rwork	0.216
R-free	0.27370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zp4
Data reduction software	XDS (Jan 31, 2020)
Data scaling software	XDS (Jan 31, 2020)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.000	47.000	2.900
High resolution limit [Å]	2.754	8.710	2.754
Rmerge	0.058	0.018	0.635
Rmeas	0.062	0.019	0.688
Rpim	0.024	0.008	0.264
Number of reflections	18363	599	2681
<I/σ(I)>	22.4
Completeness [%]	99.8	98.5	99.3
Redundancy	6.8	5.8	6.6
CC(1/2)	0.999	1.000	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	Tacsimate, PEG3350