7W5R

KRAS G12V and peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	40.556, 82.513, 82.573
Unit cell angles	119.99, 90.06, 90.02

Refinement procedure

Resolution	35.760 - 3.870
R-factor	0.2277
Rwork	0.223
R-free	0.31020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uqw
RMSD bond length	0.005
RMSD bond angle	0.992
Data reduction software	HKL-2000
Data scaling software	CCP4i
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.950
High resolution limit [Å]	3.870	3.870
Rmerge	0.088	0.184
Number of reflections	8581	2674
<I/σ(I)>	17.3
Completeness [%]	99.6
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	polyethylene glycol 3350, 0.2 M potassium nitrate at pH 6.8