7W5C

Crystal structure of Mitogen Activated Protein Kinase 4 (MPK4) from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-27
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.987
Spacegroup name	I 2 3
Unit cell lengths	132.950, 132.950, 132.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.602 - 2.201
R-factor	0.1786
Rwork	0.176
R-free	0.22540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2erk
RMSD bond length	0.010
RMSD bond angle	1.091
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.602	2.280
High resolution limit [Å]	2.201	2.201
Number of reflections	19910	1920
<I/σ(I)>	42.91
Completeness [%]	99.7
Redundancy	23.6
CC(1/2)	0.779	0.473

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	2% v/v TacsimateTM pH 7.0, 0.1 M HEPES pH 7.5, 20% w/v Polyethylene glycol 3350