7W0G

Human PPAR delta ligand binding domain in complex with a synthetic agonist H11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-19
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9789
Spacegroup name	P 1 21 1
Unit cell lengths	39.860, 93.201, 96.716
Unit cell angles	90.00, 96.27, 90.00

Refinement procedure

Resolution	28.139 - 2.443
R-factor	0.2006
Rwork	0.199
R-free	0.23650
Structure solution method	SIR
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.139	2.480
High resolution limit [Å]	2.443	2.443
Rmerge	0.096	0.096
Number of reflections	25924	25929
<I/σ(I)>	1.38
Completeness [%]	99.2
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.5M sodium citrate, pH 5.5, 19 % PEG 3350, 20% glycerol