7W05

12 mutant Ribonuclease from Hericium erinaceus GMP binding form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	34.589, 46.310, 53.524
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	11.995 - 1.290
R-factor	0.1382
Rwork	0.137
R-free	0.16620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ls1
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.310	1.210
High resolution limit [Å]	1.190	1.190
Number of reflections	22027	1444
<I/σ(I)>	33.78
Completeness [%]	98.8
Redundancy	2
CC(1/2)	1.000	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Cadmium chloride hydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 30% v/v Polyethylene glycol 400