7VQT

Crystal structure of LSD1 in complex with compound 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-16
Detector	RAYONIX MX225-HS
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	185.256, 185.256, 106.881
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.830 - 2.910
R-factor	0.2114
Rwork	0.209
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kgp
RMSD bond length	0.010
RMSD bond angle	1.336
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.830	3.090
High resolution limit [Å]	2.910	2.910
Rmeas	0.125	1.972
Rpim	0.029	0.450
Number of reflections	24188	3846
<I/σ(I)>	20.5	2.1
Completeness [%]	99.9	99.7
Redundancy	18.4	18.7
CC(1/2)	0.999	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M MES (pH 6.2-6.3), 0.2M diammonium tartrate, 0.0005M TCEP, 10-12% PEG 3350