7VPH

Crystal structure of the C-terminal tail of SARS-CoV-2 Orf6 complex with human nucleoporin pair Rae1-Nup98

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	150.657, 103.319, 136.588
Unit cell angles	90.00, 97.45, 90.00

Refinement procedure

Resolution	41.060 - 2.800
R-factor	0.1945
Rwork	0.192
R-free	0.24420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mmy
RMSD bond length	0.010
RMSD bond angle	1.380
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.065	2.850
High resolution limit [Å]	2.796	2.800
Number of reflections	51228	5087
<I/σ(I)>	1
Completeness [%]	99.3
Redundancy	3.3
CC(1/2)	0.990	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Sodium citrate pH 5.5, 20% PEG 3350