7VPG

Crystal structure of the C-terminal tail of SARS-CoV-1 Orf6 complex with human nucleoporin pair Rae1-Nup98

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	149.614, 103.299, 134.936
Unit cell angles	90.00, 95.96, 90.00

Refinement procedure

Resolution	47.440 - 2.490
R-factor	0.1867
Rwork	0.185
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mmy
RMSD bond length	0.009
RMSD bond angle	1.095
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.440	2.570
High resolution limit [Å]	2.490	2.490
Rmerge	0.165
Number of reflections	317454	7090
<I/σ(I)>	7.6
Completeness [%]	99.7
Redundancy	4.4
CC(1/2)		0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	100 mM Sodium citrate pH 5.5, 20% PEG 3350