7VN6

Crystal structure of MBP-fused BIL1/BZR1 (21-90) in complex with double-stranded DNA contaning CGCACGTGCG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2019-12-07
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	102.300, 93.608, 112.058
Unit cell angles	90.00, 100.87, 90.00

Refinement procedure

Resolution	42.750 - 2.790
R-factor	0.2182
Rwork	0.216
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zd4
RMSD bond length	0.006
RMSD bond angle	0.721
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.440	2.880
High resolution limit [Å]	1.940	1.940
Number of reflections	51574	4479
<I/σ(I)>	99.3	1.7
Completeness [%]	99.6
Redundancy	3.4
CC(1/2)	0.999	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	50 mM sodium cacodylate pH 6.5, 200 mM ammonium acetate and 10 mM calcium chloride and 10% (w/v) polyethylene glycol (PEG) 4000