7VKM
Crystal structure of TrkA (G595R) kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 105.423, 105.423, 203.741 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.690 - 2.550 |
| R-factor | 0.20184 |
| Rwork | 0.199 |
| R-free | 0.25717 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yne |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.539 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.071 | 0.456 |
| Number of reflections | 14516 | 710 |
| <I/σ(I)> | 38.25 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 3.0M NaCl, 0.1M Bis-Tris pH 6.5 |
