7VGE
Structure of the PDZ deleted variant of HtrA2 protease (S306A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-12-31 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 82.880, 82.880, 395.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.530 - 4.000 |
| R-factor | 0.26859 |
| Rwork | 0.265 |
| R-free | 0.33642 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lcy |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.497 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 4.100 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Number of reflections | 11300 | 1441 |
| <I/σ(I)> | 4.36 | 1.27 |
| Completeness [%] | 90.7 | 90.9 |
| Redundancy | 4.24 | 3.78 |
| CC(1/2) | 0.967 | 0.251 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 0.5 M Sodium acetate trihydrate pH 6.0, 2.0 M Sodium formate, 3% glycerol |
