7VG7

Plexin B1 extracellular fragment in complex with lasso-grafted PB1m6A9 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	69.190, 62.490, 82.600
Unit cell angles	90.00, 111.92, 90.00

Refinement procedure

Resolution	44.780 - 2.500
R-factor	0.1837
Rwork	0.180
R-free	0.24320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5b4w 1utg
RMSD bond length	0.010
RMSD bond angle	1.138
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (v2.8.1)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.600	2.650
High resolution limit [Å]	2.500	2.500
Rmeas	0.473
Number of reflections	22866	3621
<I/σ(I)>	6.43
Completeness [%]	99.8	99.8
Redundancy	19.7	18.5
CC(1/2)	0.975	0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M MES or HEPES, 14-16% PEG 3350