7VBT

Crystal structure of RIOK2 in complex with CQ211

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-12-20
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9897
Spacegroup name	P 21 21 21
Unit cell lengths	70.517, 93.605, 110.965
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.803 - 2.540
R-factor	0.224129822459
Rwork	0.221
R-free	0.25916
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hk6
RMSD bond length	0.004
RMSD bond angle	0.850
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.540	2.540
Number of reflections	26038	2539
<I/σ(I)>	19.8
Completeness [%]	100.0
Redundancy	8.3
CC(1/2)	0.953	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION, RECRYSTALLIZATION		293	50 mM MES (pH 5.5), 3% isopropanol, 10 mM MgCl2