7VB4
A crystal structure of alphavirus nonstructural protein 4 (nsP4) reveals an intrinsically dynamic RNA-dependent RNA polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-14 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.000, 68.290, 71.070 |
| Unit cell angles | 115.97, 106.71, 95.90 |
Refinement procedure
| Resolution | 45.000 - 1.860 |
| R-factor | 0.1881 |
| Rwork | 0.187 |
| R-free | 0.22530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7f0s |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.624 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.001 | 45.001 | 1.970 |
| High resolution limit [Å] | 1.860 | 5.530 | 1.860 |
| Rmerge | 0.062 | 0.029 | 0.912 |
| Rmeas | 0.074 | 0.034 | 1.128 |
| Total number of observations | 541271 | ||
| Number of reflections | 156684 | 6488 | 18366 |
| <I/σ(I)> | 13.2 | 40.16 | 1.27 |
| Completeness [%] | 88.4 | 96.4 | 63.9 |
| Redundancy | 3.455 | 3.381 | 2.944 |
| CC(1/2) | 0.998 | 0.998 | 0.542 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 0.2M Magnesium Acetate |
