7V9I
The Monomer mutant of BEN4 domain of protein Bend3 with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-08-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.5420 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 51.504, 61.990, 136.024 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.010 - 3.500 |
R-factor | 0.2139 |
Rwork | 0.211 |
R-free | 0.26080 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.630 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.209 | 0.461 |
Number of reflections | 2848 | 292 |
<I/σ(I)> | 6.1 | |
Completeness [%] | 95.6 | |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M Sodium formate, 20% w/v PEG 3350 |