7V91

Crystal Structure of the Catalytic Domain of a Family GH19 Chitinase from Gazyumaru, Ficus microcarpa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	90.900, 106.583, 107.656
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.094 - 1.600
Rwork	0.227
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tx7
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.061	0.381
Number of reflections	1000279	137756
<I/σ(I)>	46
Completeness [%]	99.8
Redundancy	7.3
CC(1/2)		0.937

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	3% (w/v) gamma-polyglutamic acid low molecule, 25%(w/v) 2-Methy-2, 4-pentanediol, 0.1 M HEPES (pH 7.5), 0.5 M Ammonium sulfate