7V6F

Structure of Candida albicans Fructose-1,6-bisphosphate aldolase complexed with G3P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	119.458, 56.570, 131.747
Unit cell angles	90.00, 113.43, 90.00

Refinement procedure

Resolution	43.680 - 2.980
R-factor	0.2204
Rwork	0.217
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lnk
RMSD bond length	0.006
RMSD bond angle	1.490
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.680	2.988
High resolution limit [Å]	2.885	2.885
Number of reflections	18393
<I/σ(I)>	22.7
Completeness [%]	99.5
Redundancy	6.7
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	100 mM Hepes pH =7.5, 11% (w/v) PEG 3350