7V4P

Unique non-heme hydroxylase in biosynthesis of nucleoside antibiotic pathway uncover mechanism of reaction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	80
Detector technology	CCD
Collection date	2020-11-20
Detector	RAYONIX MX300HE
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	82.083, 82.083, 104.172
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.460 - 1.950
R-factor	0.1917
Rwork	0.188
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p7x
RMSD bond length	0.011
RMSD bond angle	1.270
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.460	2.020
High resolution limit [Å]	1.950	1.950
Number of reflections	26710	26733
<I/σ(I)>	36.9
Completeness [%]	100.0
Redundancy	15.7
CC(1/2)	0.939	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Sodium acetate 0.1M Bis Tris propane pH 6.5 20 % W/V PEG 3350