7V39

Crystal structure of NP exonuclease-PCMB complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-04
Detector	RAYONIX MX300HE
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	50.185, 76.292, 140.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.816 - 2.200
R-factor	0.2018
Rwork	0.200
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7v37
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.280
High resolution limit [Å]	2.200	4.730	2.200
Rmerge	0.089	0.053	0.395
Rmeas	0.097	0.058	0.432
Rpim	0.038	0.023	0.173
Total number of observations	179251
Number of reflections	28303	3033	2793
<I/σ(I)>	11.8
Completeness [%]	99.9	99.8	100
Redundancy	6.3	6.2	6.1
CC(1/2)		0.998	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M DL-Malic acid pH 7.0, 20% w/v Polyethylene glycol 3,350, 2mM Sodium p-hydroxymercuribenzoate