7V37

Crystal structure of apo-NP exonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97622
Spacegroup name	I 2 2 2
Unit cell lengths	74.129, 121.276, 137.722
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.739 - 2.403
R-factor	0.2109
Rwork	0.208
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g9z
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.490
High resolution limit [Å]	2.400	5.160	2.400
Rmerge	0.101	0.046	0.494
Rmeas	0.113	0.052	0.574
Rpim	0.050	0.022	0.285
Total number of observations	111142
Number of reflections	24007	2487	2333
<I/σ(I)>	7.5
Completeness [%]	98.8	96.9	98.3
Redundancy	4.6	5.1	3.7
CC(1/2)		0.998	0.723

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Sodium citrate tribasic dihydrate pH 5.5, 38% v/v Polyethylene glycol 200, 10 mM Aurintricarboxylic acid