7V1A
Stapled TBS peptide from RIAM bound to talin R7R8 domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 60.864, 104.334, 48.233 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.572 - 1.845 |
R-factor | 0.2175 |
Rwork | 0.215 |
R-free | 0.25380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4w8p |
Data reduction software | HKL-2000 |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 104.330 | 104.330 | 1.880 |
High resolution limit [Å] | 1.845 | 9.040 | 1.850 |
Rmerge | 0.033 | 0.022 | 1.546 |
Rmeas | 0.036 | 0.024 | 1.689 |
Rpim | 0.014 | 0.011 | 0.672 |
Total number of observations | 1507 | 9226 | |
Number of reflections | 27043 | 288 | 1512 |
<I/σ(I)> | 22.6 | 59.6 | 1 |
Completeness [%] | 99.4 | 98.3 | 91.9 |
Redundancy | 6.5 | 5.2 | 6.1 |
CC(1/2) | 0.999 | 0.999 | 0.475 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | 100 mM NaCl, 20% (w/v) polyethylene glycol 3350 and 20% (v/v) ethylene glycol. |