7UZC
Structure of the SARS-CoV-2 RBD in complex with the mouse antibody Fab fragment, M8a-34
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.470, 165.165, 93.715 |
| Unit cell angles | 90.00, 106.63, 90.00 |
Refinement procedure
| Resolution | 39.230 - 2.200 |
| R-factor | 0.1733 |
| Rwork | 0.172 |
| R-free | 0.24330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7sc1 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.450 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.120 | 1.611 |
| Rmeas | 0.130 | 1.746 |
| Rpim | 0.048 | 0.663 |
| Number of reflections | 79165 | 4396 |
| <I/σ(I)> | 9.9 | 1.2 |
| Completeness [%] | 98.6 | 96.4 |
| Redundancy | 7 | 6.7 |
| CC(1/2) | 0.997 | 0.608 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2% v/v tacsimate pH 4.0, 0.1 M sodium acetate trihydrate pH 4.6, 16 % PEG 3350 |
