7UZC

Structure of the SARS-CoV-2 RBD in complex with the mouse antibody Fab fragment, M8a-34

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	54.470, 165.165, 93.715
Unit cell angles	90.00, 106.63, 90.00

Refinement procedure

Resolution	39.230 - 2.200
R-factor	0.1733
Rwork	0.172
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7sc1
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.450	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.120	1.611
Rmeas	0.130	1.746
Rpim	0.048	0.663
Number of reflections	79165	4396
<I/σ(I)>	9.9	1.2
Completeness [%]	98.6	96.4
Redundancy	7	6.7
CC(1/2)	0.997	0.608

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2% v/v tacsimate pH 4.0, 0.1 M sodium acetate trihydrate pH 4.6, 16 % PEG 3350