7UW0

Crystal structure of human ClpP protease in complex with TR-133

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	I 1 2 1
Unit cell lengths	105.660, 153.798, 133.208
Unit cell angles	90.00, 107.03, 90.00

Refinement procedure

Resolution	51.690 - 2.800
R-factor	0.2623
Rwork	0.260
R-free	0.30880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bba
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.620	2.890
High resolution limit [Å]	2.800	2.800
Number of reflections	47509	4706
<I/σ(I)>	4.2
Completeness [%]	95.2
Redundancy	4.9
CC(1/2)	0.805	0.420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 sodium acetate, pH 4.6 to 5.2, 5% PEG 4000