7UUO

Crystal structure of aminoglycoside resistance enzyme ApmA H135A mutant, complex with tobramycin and coenzyme A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 3
Unit cell lengths	108.292, 108.292, 87.726
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.450 - 2.650
R-factor	0.2877
Rwork	0.286
R-free	0.32820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jm2
RMSD bond length	0.006
RMSD bond angle	1.082
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.144	1.610
Rpim	0.052	0.605
Number of reflections	33452	1674
<I/σ(I)>	16.7	1.43
Completeness [%]	99.9	99.9
Redundancy	8.1	7.5
CC(1/2)	0.998	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	298	0.1 M sodium acetate pH 4.5, 10% PEG 10K, 2 mM tobramycin