7UK1

Complex Structure of Human Polypyrimidine Splicing Factor (PSF/SFPQ) with Murine Virus-like 30S Transcript-1 (VS30-1) Reveals Cooperative Binding of RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-10-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	61.451, 64.658, 68.837
Unit cell angles	90.00, 97.79, 90.00

Refinement procedure

Resolution	48.870 - 2.700
R-factor	0.2045
Rwork	0.200
R-free	0.29100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wii
RMSD bond length	0.008
RMSD bond angle	1.680
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.870	2.750
High resolution limit [Å]	2.690	2.690
Rmeas	0.910
Rpim	0.129
Number of reflections	17804	728
<I/σ(I)>	17.1
Completeness [%]	98.9
Redundancy	44.1
CC(1/2)		0.125

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	285.15	0.1 M MgCl2, 0.1 M HEPES pH 7.0, 15% (w/v),PEG4000, or 15% (w/v) PEG3500, or 20% PEG2000MME