7UGE

Bromodomain of CBP liganded with BMS-536924

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	93
Detector technology	CCD
Collection date	2019-08-20
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	43.122, 59.293, 57.147
Unit cell angles	90.00, 109.81, 90.00

Refinement procedure

Resolution	39.829 - 2.004
R-factor	0.1665
Rwork	0.164
R-free	0.19710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7kpy
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.830	2.030
High resolution limit [Å]	2.000	2.000
Rmeas	0.121	0.382
Number of reflections	17935	894
<I/σ(I)>	16.1	3.3
Completeness [%]	97.9
Redundancy	3.5
CC(1/2)	0.999	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.2M Lithium sulfate monohydrate, 0.1M BIS-TRIS pH6.5, 25% w/v Polyethylene glycol 3,350