7UAJ
Crystal structure of apo HPV16 E6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-11-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 |
Unit cell lengths | 84.110, 88.680, 109.610 |
Unit cell angles | 106.61, 90.16, 102.76 |
Refinement procedure
Resolution | 52.390 - 3.250 |
R-factor | 0.2572 |
Rwork | 0.256 |
R-free | 0.28240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xr8 |
Data reduction software | MOSFLM (v 7.3.0) |
Data scaling software | Aimless (v 0.7.4) |
Phasing software | PHASER (v 2.8.3) |
Refinement software | PHENIX (v 1.20.1-4887-000) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 82.670 | 82.670 | 3.370 |
High resolution limit [Å] | 3.250 | 12.160 | 3.250 |
Rmerge | 0.123 | 0.054 | 1.689 |
Rmeas | 0.144 | 0.067 | 1.960 |
Rpim | 0.075 | 0.040 | 0.994 |
Total number of observations | 159101 | 3169 | 17073 |
Number of reflections | 42715 | 857 | 4578 |
<I/σ(I)> | 5.7 | 13.6 | 0.9 |
Completeness [%] | 91.9 | 97.2 | 93.3 |
Redundancy | 3.7 | 3.7 | 3.7 |
CC(1/2) | 0.919 | 0.886 | 0.401 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M HEPES, 8% ethylene glycol and 10% PEG 8000 at pH 7.5 |