7U6P

Structure of an intellectual disability-associated ornithine decarboxylase variant G84R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	74.806, 85.879, 154.738
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.464 - 2.350
R-factor	0.1907
Rwork	0.188
R-free	0.22450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7s3f
RMSD bond length	0.003
RMSD bond angle	0.653
Data reduction software	MOSFLM
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.600	2.430
High resolution limit [Å]	2.350	2.350
Rmerge		1.350
Number of reflections	42293	4104
<I/σ(I)>	8.5
Completeness [%]	99.9
Redundancy	9.4
CC(1/2)	0.993	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	0.2 M sodium acetate trihydrate pH 7.0, 20% w/v polyethylene glycol 3350